Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10941034 | Immunobiology | 2013 | 8 Pages |
Abstract
An altered redox status and increased lymphocyte apoptosis have been implicated in the development of systemic lupus erythematosus (SLE). In this study, we evaluated the relationship between glutathione (GSH) depletion, reactive oxygen species (ROS) and, the progression of apoptosis and their association with SLE severity. Significant low levels of intracellular glutathione, total thiol and altered redox state (GSH/GSSG) were found in SLE patients, in which lymphocyte apoptosis and activated caspase-3 expression in the lymphocytes were remarkably increased. The severity of disease was positively allied with the increased levels of lymphocyte apoptosis and caspase-3, but negatively with the decreased levels of total thiol, depleted intracellular glutathione and altered redox state (GSH/GSSG). The lymphocyte apoptosis and activated caspase-3 expression were negatively associated with intracellular levels of GSH and redox state and positively associated with the elevated levels of multiple oxidative stress markers; ROS and lipid peroxidation measured as malondialdehyde (MDA). These results suggest that GSH depletion and elevated oxidative stress trigger apoptosis and may be coupled with the severity of the disease.
Keywords
PBMCsNBTLPODCFH-DAGPXGSHTSHCATMDAMFIFITCESR2′,7′-dichlorofluorescein diacetateROSnitroblue tetrazoliumTotal thiolscomplement component 3complement component 4ApoptosisSODerythrocyte sedimentation rateperipheral blood mononuclear cellsSuperoxide dismutasefluorescein isothiocyanateSystemic lupus erythematosusSystemic lupus erythematosus (SLE)SLEmalondialdehydemean fluorescence intensityLipid peroxidationPropidium iodideCatalasereduced glutathioneGlutathioneglutathione reductaseglutathione peroxidaseReactive oxygen species
Related Topics
Life Sciences
Biochemistry, Genetics and Molecular Biology
Cell Biology
Authors
Dilip Shah, Sangita Sah, Ajay Wanchu, Mei X. Wu, Archana Bhatnagar,