Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10953705 | Journal of Molecular and Cellular Cardiology | 2015 | 5 Pages |
Abstract
Macrophages, a significant component of atherosclerotic plaques vulnerable to acute complications, can be pro-inflammatory (designated M1), regulatory (M2), lipid- (Mox) or Heme-induced (Mhem). We showed previously that low (LSS) and oscillatory (OSS) shear stress cause thin-cap fibroatheroma and stable smooth muscle cell-rich plaque formation respectively in ApoE-knockout (ApoEâ/â) mice. Here we investigated whether different shear stress conditions relate to specific changes in macrophage polarization and plaque morphology by applying a shear stress-altering cast to the carotid arteries of high fat-fed ApoEâ/â mice. The M1 markers iNOS and IRF5 were highly expressed in macrophage-rich areas of LSS lesions compared to OSS lesions 6Â weeks after cast placement, while the M2 marker Arginase-1, and Mox/Mhem markers HO-1 and CD163 were elevated in OSS lesions. Our data indicates shear stress could be an important determinant of macrophage polarization in atherosclerosis, with low shear promoting M1 programming.
Keywords
IRF5T helper 1IL-13Th1IL-12LSSHeme oxygenase-1TCFAIFN-γIL-10iNOSHO-1IL-4ApoE−/−thin-cap fibroatheromaAtherosclerosisOscillatory shear stressOSSInterleukin 10interleukin 12interleukin 13Interleukin 4Shear stresslow shear stresstumor necrosis factor alphainducible nitric oxide synthaseinterferon regulatory factor 5TNF-αMacrophage8-hydroxyguanosineInterferon gamma
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Authors
Anusha N. Seneviratne, Jennifer E. Cole, Michael E. Goddard, Inhye Park, Zahra Mohri, Stephen Sansom, Irina Udalova, Rob Krams, Claudia Monaco,