Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10953734 | Journal of Molecular and Cellular Cardiology | 2015 | 31 Pages |
Abstract
Recent advances in sequencing and genomic technologies have resulted in the discovery of thousands of previously unannotated long noncoding RNAs (lncRNAs). However, their function in the cardiovascular system remains elusive. Here we review and discuss considerations for cardiovascular lncRNA discovery, annotation and functional characterization. Although we primarily focus on the heart, the proposed pipeline should foster functional and mechanistic exploration of these transcripts in various cardiovascular pathologies. Moreover, these insights could ultimately lead to novel therapeutic approaches targeting lncRNAs for the amelioration of cardiovascular diseases including heart failure.
Keywords
lncRNAsRT-PCRlincRNAsSMCGTFRNA-seqChIP-Seqlong noncoding RNAscardiovascular diseaseES cellSmooth muscle cellEmbryonic stem cellGene regulatory networkchirpLong noncoding RNAMass spectrometryTranscription factorCardiomyocyteGenome-wide association studyGWASMicroRNAMiRNAheart failurereverse-transcription polymerase chain reactionFunctional characterizationChartGenomicsCLASHDiscoveryGRN
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Authors
Samir Ounzain, Frédéric Burdet, Mark Ibberson, Thierry Pedrazzini,