| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10953881 | Journal of Molecular and Cellular Cardiology | 2012 | 11 Pages |
Abstract
⺠The role of mitochondrial tubulin beta II and its role in respiration regulation are described. ⺠Mitochondrial tubulin beta II is rapidly removed by short proteolytic treatment of permeabilized cardiomyocytes. ⺠Removal of tubulin increases the permeability of outer mitochondrial membrane for ADP. ⺠Quality tests for isolated cardiomyocytes are described.
Keywords
MtCKPEPANTVDACDTTSTIIMSATRPBSBSAatractylosidebovine serum albuminadenine nucleotide translocasetubulinEnergy fluxesdithiothreitolWashing solutionmitochondrial outer membraneMitochondrial inner membranePhosphocreatinephosphoenolpyruvateMitochondrial intermembrane spaceCardiomyocytesMoMphosphate buffer solutionIsolation mediumMIMsoybean trypsin inhibitorMitochondriaPCRpyruvate kinaseCytoskeletonvoltage-dependent anion channelcreatineCreatine kinaseMitochondrial creatine kinase
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Authors
Marcela Gonzalez-Granillo, Alexei Grichine, Rita Guzun, Yves Usson, Kersti Tepp, Vladimir Chekulayev, Igor Shevchuk, Minna Karu-Varikmaa, Andrey V. Kuznetsov, Michael Grimm, Valdur Saks, Tuuli Kaambre,