Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10956131 | Molecular and Cellular Endocrinology | 2014 | 10 Pages |
Abstract
Recent advances in establishing the role of the aryl hydrocarbon receptor (Ahr) in normophysiology have discovered its fundamental role, amongst others, in female reproduction. Considering previous studies suggesting the hormonal modulation of Ahr, we aimed to investigate whether in murine granulosa cells (GCs) the gonadotropins regulate Ahr expression and how this is mechanistically implemented. We found that the FSH-like substance - pregnant mare serum gonadotropin - led to stimulation of Ahr expression. More importantly hCG produced relatively rapid reduction of Ahr mRNA in GCs of preovulatory follicles. We show for the first time that LHCGR signaling in regulating the Ahr message involves protein kinase A pathway and is attributable to decreased transcription rate. Finally, we found that Ahr promoter accessibility was decreased by hCG, implicating chromatin remodeling in Ahr gene regulation by LH.
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Authors
Indrek Teino, Antti Matvere, Sulev Kuuse, Sulev Ingerpuu, Toivo Maimets, Arnold Kristjuhan, Tarmo Tiido,