Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10956195 | Molecular and Cellular Endocrinology | 2014 | 11 Pages |
Abstract
Wnt signaling is an evolutionarily conserved pathway that regulates cell proliferation, differentiation and apoptosis. To investigate the possible role of Wnt signaling in the regulation of ovarian follicular development, secondary follicles were isolated and cultured in vitro in the presence or absence of its activator (LiCl or Wnt3a) or inhibitor (IWR-1). We have demonstrated that activation of β-catenin signals by activators dramatically suppressed follicular development by increasing granulosa cell apoptosis and inhibiting follicle steroidogenesis. In contrast, inhibition of Wnt signaling by IWR-1 was observed with better developed follicles and increased steroidogenesis. Further studies have shown that the transcription factor Forkhead box O3a (Foxo3a) and its downstream target molecules were modulated by the activators or the inhibitor. These findings provide evidence that Wnt signaling might negatively regulate follicular development potentially through Foxo3a signaling components.
Keywords
WntIWRCytochrome P450 aromataseIWR-1p53-upregulated modulator of apoptosisCYP19A1CYP11A13β-Hydroxysteroid dehydrogenaseWnt3afrizzledFZDCTNNB1terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labelingFBSGSK3βLiClFOXO3aLRP3β-HSDAPCDMSOTCF/LEFadenomatosis polyposis coliβ-cateninTUNELforkhead Box ODimethylsulfoxideStarfetal bovine serumWnt/β-catenin signalingFoxOfollicle cultureSteroidogenic acute regulatory proteinPUMAGlycogen synthase kinase 3β
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Authors
Lei Li, Shao-Yang Ji, Jun-Ling Yang, Xi-Xia Li, Jun Zhang, Yang Zhang, Zhao-Yuan Hu, Yi-Xun Liu,