mTOR inhibition with rapamycin causes impaired insulin signalling and glucose uptake in human subcutaneous and omental adipocytes

Article ID	Journal	Published Year	Pages	File Type
10956235	Molecular and Cellular Endocrinology	2012	10 Pages	PDF

Abstract

âº Effects of rapamycin in human subcutaneous and omental adipocytes on glucose metabolism. âº Therapeutic rapamycin concentration reduces basal and insulin stimulated glucose uptake. âº Reduced glucose uptake is associated with impaired insulin signalling. âº Rapamycin effects on adipocytes may contribute to therapy associated diabetes.

Keywords

PI3K bicinchoninic acid protein SIN1 p70S6K1 mTORC2 PDK1 mTORC1 PKB mTOR BCA ECL TSC p70 ribosomal S6 kinase Adipocytes Pest enhanced chemiluminescence Glucose uptake Rapamycin Insulin signalling Immunosuppressive agents Phosphatidylinositol 3-kinase NODAT mammalian target of rapamycin Mammalian target of rapamycin complex 2 Mammalian target of rapamycin complex 1 phosphoinositide-dependent kinase 1 protein kinase B penicillin–streptomycin Tuberous sclerosis complex insulin receptor