Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10956341 | Molecular and Cellular Endocrinology | 2011 | 8 Pages |
Abstract
Gene or genome duplication is a fundamental evolutionary mechanism leading towards the origin of new genes, or gene functions. Myostatin (MSTN) is a negative regulator of muscle growth that in teleost fish, as a result of genome duplication, is present in double copy. This study provides evidence of differentiation of MSTN paralogs in fish by comparatively exploring their tissue-regulation in the Asian sea bass (Lates calcarifer) when subjected to fasting stress. Results showed differential regulation as well as specific tissue-responses in the muscle, liver, gill and brain of L. calcarifer after nutritional deprivation. In particular, the LcMstn-1 expression increased in liver (â¼4 fold) and muscle (â¼3 fold) and diminished in brain (â¼0.5 fold) and gill (â¼0.5 fold) while that of LcMstn-2 remained stable in brain and muscle and was up regulated in gill (â¼2.5 fold) and liver (â¼2 fold). Differential regulation of Mstn paralogs was supported by in silico analyses of regulatory motifs that revealed, at least in the immediate region upstream the genes, a differentiation between Mstn-1 and Mstn-2. The Mstn-1 in particular showed a significantly higher conservation of regulatory sites among teleost species compared to its paralog indicating that this gene might have a highly conserved function in the taxon.
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Authors
C. De Santis, D.R. Jerry,