Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10956905 | Molecular and Cellular Neuroscience | 2005 | 8 Pages |
Abstract
The tripartite motif proteins TRIM-2 and TRIM-3 have been put forward as putative organizers of neuronal outgrowth and structural plasticity. Here, we identified a molluscan orthologue of TRIM-2/3, named L-TRIM, which is up-regulated during in vitro neurite outgrowth of central neurons. In adult animals, L-Trim mRNA is ubiquitously expressed at low levels in the central nervous system and in peripheral tissues. Central nervous system expression of L-Trim mRNA is increased during postnatal brain development and during in vitro and in vivo neuronal regeneration. In vitro double-stranded RNA knock-down of L-Trim mRNA resulted in a >70% inhibition of neurite outgrowth. Together, our data establish a crucial role for L-TRIM in developmental neurite outgrowth and functional neuronal regeneration and indicate that TRIM-2/3 family members may have evolutionary conserved functions in neuronal differentiation.
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Authors
M.T. van Diepen, G.E. Spencer, J. van Minnen, Y. Gouwenberg, J. Bouwman, A.B. Smit, R.E. van Kesteren,