Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10956998 | Molecular and Cellular Neuroscience | 2005 | 13 Pages |
Abstract
We previously showed that deletion of the cell surface molecule mCD24 resulted in an increased proliferation in adult subventricular zone (SVZ). Here, we report an increased PSA-NCAM+/TuJ1â population in the mCD24â/â in vivo SVZ as well as in vitro neurospheres. Isolated in vitro, these cells were able to generate neurospheres. Proliferation studies, using BrdU incorporation, showed an increased proliferation in P7 mCD24â/â SVZ and neurospheres. Using electron microscopy, the same cell types were identified in the in vivo SVZ as well as in vitro neurospheres from the WT and mCD24â/â mice. In mixed neurospheres, formed with WT and EGFP/KO cells (enhanced green fluorescent protein mCD24â/â), the WT environment was able to control the proliferation rate of the mCD24â/â cells, but was unable to regulate their differentiation. We concluded that mCD24 acts cell nonautonomously to regulate transit-amplifying cells proliferation and/or differentiation.
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Authors
Vincent Nieoullon, Richard Belvindrah, Geneviève Rougon, Geneviève Chazal,