Moderate superoxide production is an early promoter of mitochondrial biogenesis in differentiating N2a neuroblastoma cells

Article ID	Journal	Published Year	Pages	File Type
10958062	Neurochemistry International	2012	11 Pages	PDF

Abstract

âº Differentiation of N2a cells is associated with an increase in mitochondrial mass. âº An early, moderate increase in superoxide is observed in differentiating cells. âº O2â scavenging prevents mitochondrial biogenesis and differentiation. âº MAPK inhibition prevents mitochondrial biogenesis and differentiation. âº Mitochondriogenesis and differentiation are part of a O2â mediated hormetic response.

Keywords

pKa MitoTracker Green dibutyryl cyclic AMP Mitohormesis PKI MnTBAP PGC-1α forskolin CREB MTG PBS 2′,7′-dichlorodihydrofluorescein diacetate ERK1/2 H2DCFDA MAPK MTT O2−ROS superoxide anion Neural differentiation Mitochondrial biogenesis Superoxide phosphate buffer saline Protein kinase A inhibitor Mitochondriogenesis cAMP responsive element binding protein protein kinase A Propidium iodide extracellular signal-regulated kinase mitogen-activated protein kinases Reactive oxygen species