Guanosine protects human neuroblastoma SH-SY5Y cells against mitochondrial oxidative stress by inducing heme oxigenase-1 via PI3K/Akt/GSK-3Î² pathway

Article ID	Journal	Published Year	Pages	File Type
10958137	Neurochemistry International	2012	8 Pages	PDF

Abstract

âº Guanosine protects SH-SY5Y cells from oxidative damage induced by rotenone plus oligomycin. âº Guanosine promotes neuroprotection involves modulation of A1, A2A adenosine receptors and BK channels activation. âº Guanosine-induced neuroprotection involves activation of the PI3K-Akt pathway. âº Guanosine promotes GSK-3Î² inhibition. âº Guanosine is protective via induction of the antioxidant enzyme heme oxygenase-1.

Keywords

DPCPX 4-(4-fluorophenyl)-2-(4-methylsulfinylphenyl)-5-(4-pyridyl)-1H-imidazole 2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one ZM241385 SB203580 PD98059 LY294002 Heme oxygenase-1 PKC GSK-3β HO-1 PI3K 1,3-Dipropyl-8-cyclopentylxanthine 2′,7′-dichlorofluorescein diacetate 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide H2DCFDA MAPK MTT ROS SnPP Akt phosphorylation MEK Neuroprotection Protein kinase C mitogen-activated protein kinase mitogen-activated protein kinase kinase Glycogen synthase kinase 3β Guo guanosine Reactive oxygen species