SIRT2 interferes with autophagy-mediated degradation of protein aggregates in neuronal cells under proteasome inhibition

Article ID	Journal	Published Year	Pages	File Type
10958211	Neurochemistry International	2012	9 Pages	PDF

Abstract

âº SIRT2 is a class III histone deacetylse that is abundantly expressed in aging brain tissue. âº SIRT2 aggravates MG132-induced accumulation of ubiquitinated proteins. âº SIRT2 inhibits perinuclear aggresome formation and autophagic flux under proteasome inhibition.

Keywords

PEI Aggresome LC3 SIRT2 ECL HDAC Aβ CMA FBS DMEM 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide Chaperone-mediated autophagy BSA MTT bovine serum albumin Autophagy amyloid beta enhanced chemiluminescence Alzheimer’s disease Huntington’s disease Parkinson’s disease Protein accumulation Neurodegeneration Retinoic acid fetal bovine serum ubiquitin–proteasome system LAMP-2 Dulbecco’s modified eagle’s medium histone deacetylase lysosomal-associated membrane protein 2 microtubule-associated protein 1 light chain 3 polyethyleneimine UPS