Role of the phosphatidylinositol 3-kinase and extracellular signal-regulated kinase pathways in the neuroprotective effects of cilnidipine against hypoxia in a primary culture of cortical neurons

Article ID	Journal	Published Year	Pages	File Type
10958230	Neurochemistry International	2012	11 Pages	PDF

Abstract

âº Hypoxia induces neuronal cell death through various mechanisms. âº The PI3K and ERK pathways are important for neuronal survival. âº Cilnidipine is a calcium channel blocker. âº Cilnidipine has neuroprotective effects against hypoxia. âº The PI3K and ERK pathways play important role in its neuroprotective effects.

Keywords

PI3K Tris-buffered saline containing 0.05% Tween-20 DHP CCBs 2′7′-dichlorofluorescein pAkt DCFH2 GSK-3 TBST PMSF IRS HBSS DAPI DCFH-DA ERK TBS Dcf PBS COX 2′,7′-dichlorodihydrofluorescein DMSO Antioxidant cyclooxygenase sodium dodecyl sulfate–polyacrylamide gel electrophoresis SDS-PAGE terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling TUNEL Dihydropyridine dichlorodihydrofluorescein diacetate Dimethyl sulfoxide Stroke Phosphate buffered saline Phosphatidylinositol 3-kinase phosphorylated Akt Phenylmethylsulfonylfluoride lactate dehydrogenase LDH Hank’s balanced salt solution Calcium channel blockers Calcium channel blocker Hypoxia extracellular signal-related kinase glycogen synthase kinase-3