A self-propelling cycle mediated by reactive oxide species and nitric oxide exists in LPS-activated microglia

Article ID	Journal	Published Year	Pages	File Type
10958235	Neurochemistry International	2012	11 Pages	PDF

Abstract

âº Inducible nitric oxide synthase (iNOS) mediated the NO production in LPS-stimulated microglia. âº NADPH oxidase mediated the iROS production in LPS-stimulated microglia. âº NO promoted iROS production through up-regulate the NADPH oxidase expression in LPS-stimulated microglia. âº IROS promoted NO production through up-regulate the iNOS expression in LPS-stimulated microglia. âº A self-propelling cycle mediated by reactive oxide species and nitric oxide exists in LPS-activated microglia.

Keywords

NADPH MDM N-nitro-l-arginine methyl ester IFN-γ iROS IL-1β HBSS DCFH-DA iNOS NBT DPI NAC CAT NF-κB LPS FBS POD DMSO l-NAME MAPKs MTT N-acetylcysteine Small interfering RNA siRNA Iscove’s Modified Dulbecco’s Medium NADPH oxidase interferon-γ Interleukin-1β Nitro blue tetrazolium chloride tumor necrosis factor-α CNS Dimethylsulfoxide SOD fetal bovine serum inducible nitric oxide synthase Superoxide dismutase central nervous system TNF-α nuclear factor-κB lipopolysaccharide methyl thiazolyl tetrazolium Hank’s balanced salt solution Microglia sodium nitroprusside Nitric oxide nicotinamide adenine dinucleotide phosphate oxidase Peroxidase SNP Catalase