Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10958355 | Neurochemistry International | 2010 | 8 Pages |
Abstract
Parkinson's disease and other neurodegenerative disorders associated to changes in α-synuclein often result in autonomic dysfunction, most of the time accompanied by abundant expression of this synaptic protein in peripheral autonomic neurons. Given that expression of α-synuclein in vascular elements has been previously reported, the present study was undertaken to determine whether α-synuclein directly participates in the regulation of vascular responsiveness. We detected by immunohistochemistry perivascular nerve fibers containing α-synuclein in the aorta of mice while aortic endothelial cells and muscular fibers themselves did not exhibit detectable levels of this protein. To assess the effect of α-synuclein on vascular reactivity, aortic ring preparations obtained from α-synuclein-deficient knockout mice and from transgenic mice overexpressing human wild-type α-synuclein under the control of the tyrosine hydroxylase-promoter were mounted and equilibrated in organ baths for isometric tension recording. Lack of α-synuclein did not modify the relaxant responses to the endothelium-dependent (acetylcholine) and -independent (sodium nitroprusside) vasodilators, but resulted in a greater than normal norepinephrine-induced vasoconstriction along with a lowered response to dopamine, suggesting potential presynaptic changes in dopamine and norepinephrine releases in knockout mice. Overexpression of α-synuclein in TH-positive fibers resulted in complex abnormal responses, characterized by lowered acetylcholine-induced relaxation and lowered norepinephrin-induced contraction. Taken together, our data show for the first time that α-synuclein is present in sympathetic fibers supplying the murine aorta and provide evidence that changes in α-synuclein levels in perivascular fibers play a physiological role in the regulation of vascular function.
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Authors
Vannina G. Marrachelli, Francisco J. Miranda, José A. AlabadÃ, Miguel Milán, Marifé Cano-Jaimez, Martina Kirstein, Enrique Alborch, Isabel Fariñas, Francisco Pérez-Sánchez,