Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10962602 | Vaccine | 2016 | 7 Pages |
Abstract
Infectious bursal disease virus (IBDV) causes immunosuppression in young chickens, leading to increased susceptibility to other diseases and a reduction in the immune response to other vaccines. Thus, IBDV results in great economic losses to the poultry industry. The most effective method of prevention is vaccination. However, medium-virulence vaccines can cause bursal pathological damage and immunosuppression. Here, we describe a safer, self-assembled, subvirus-like particle (sVP) vaccine without a complex purification process. The IBD-VP2 gene was cloned into Pichia pastoris, and the expressed protein self-assembled into TÂ =Â 1 sVPs (â¼23Â nm). Immunization experiments showed that the sVP vaccine elicited high IBDV-neutralizing antibodies in each group, and all birds survived challenge with very virulent IBDV (vvIBDV). Additionally, IBDV RNA was not detected, and sterile immunity was achieved. In conclusion, the IBD-sVP is a suitable candidate for a recombinant subunit vaccine against IBDV.
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Authors
Miao Wang, Qing Pan, Zhen Lu, Kai Li, Honglei Gao, Xiaole Qi, Yulong Gao, Xiaomei Wang,