| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10962760 | Vaccine | 2016 | 6 Pages |
Abstract
Subcutaneous lethal dose 50 (LD50) of AahG50 and BotG50 is of 575 μg/kg and 1300 μg/kg respectively. By comparison, BotG50/PLA is totally innocuous while 50% of tested mice survive 2875 μg AahG50/kg. Alhydrogel and Freund are not able to detoxify such a high dose. Cross-antigenicity between particulate and soluble fraction is also, ensured. AahG50/PLA and BotG50/PLA induce high antibody levels in mice serum. The neutralizing capacity per mL of anti-venom was 258 μg/mL and 186 μg/mL calculated for anti-AahG50/PLA and anti-BotG50/PLA sera, respectively. Animals immunized with AahG50/PLA are protected against AahG50 injected dose of 3162 μg/kg as opposed all non-immunized mice died at this dose. We find that the detoxification approach based PLA nanoparticles, benefit the immunogenicity and protective efficacy of venom immunogen.
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Immunology
Authors
Sana Ayari-Riabi, Thomas Trimaille, Kamel Mabrouk, Denis Bertin, Didier Gigmes, Zakaria Benlasfar, Ahlem Zaghmi, Balkiss Bouhaouala-Zahar, Mohamed Elayeb,