Competitive detection of influenza neutralizing antibodies using a novel bivalent fluorescence-based microneutralization assay (BiFMA)

- We have developed a bivalent fluorescence assay to identify influenza neutralizing antibodies.
- The bivalent nature of the approach reduces by half the time, reagents, and sera needed.
- Direct competition of antibody neutralization against disparate HAs can be evaluated.
- Rapid and safe methodology amenable to facilities lacking BSL-3 capabilities.
- Can be used to identify and characterize influenza broadly neutralizing antibodies.