Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10963382 | Vaccine | 2015 | 6 Pages |
Abstract
Zoonoses that affect human and animal health have an important economic impact. In the study now presented, a bivalent vaccine has been developed that has the potential for preventing the transmission from cattle to humans of two bacterial pathogens: Brucella abortus and Shiga toxin-producing Escherichia coli (STEC). A 66 kDa chimeric antigen, composed by EspA, Intimin, Tir, and H7 flagellin (EITH7) from STEC, was constructed and expressed in B. abortus Îpgm vaccine strain (BabÎpgm). Mice orally immunized with BabÎpgm(EITH7) elicited an immune response with the induction of anti-EITH7 antibodies (IgA) that clears an intestinal infection of E. coli O157:H7 three times faster (t = 4 days) than mice immunized with BabÎpgm carrier strain (t = 12 days). As expected, mice immunized with BabÎpgm(EITH7) strain also elicited a protective immune response against B. abortus infection. A Brucella-based vaccine platform is described capable of eliciting a combined protective immune response against two bacterial pathogens with diverse lifestyles-the intracellular pathogen B. abortus and the intestinal extracellular pathogen STEC.
Related Topics
Life Sciences
Immunology and Microbiology
Immunology
Authors
Florencia Iannino, Claudia K. Herrmann, Mara S. Roset, Gabriel Briones,