Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10965169 | Vaccine | 2014 | 6 Pages |
Abstract
We explored the possibilities for purification of various ÎNS1 live, replication deficient influenza viruses on ion exchange methacrylate monoliths. Influenza A ÎNS1-H1N1, ÎNS1-H3N2, ÎNS1-H5N1 and ÎNS1-influenza B viruses were propagated in Vero cells and concentrated by tangential flow filtration. All four virus strains adsorbed well to CIM QA and CIM DEAE anion exchangers, with CIM QA producing higher recoveries than CIM DEAE. ÎNS1-influenza A viruses adsorbed well also to CIM SO3 cation exchanger at the same pH, while ÎNS1-influenza B virus adsorption to CIM SO3 was not complete. Dynamic binding capacity (DBC) for CIM QA, DEAE and SO3 methacrylate monoliths for influenza A ÎNS1-H1N1 virus were 1.9E + 10 TCID50/ml, 1.0E + 10 TCID50/ml and 8.9E + 08 TCID50/ml, respectively. Purification of ÎNS1 viruses on CIM QA was scaled up and reproducibility was confirmed. Yields of infectious virus on CIM QA were between 70.8 ± 32.3% and 87 ± 30.8%. Total protein removal varied from 93.3 ± 0.4% to 98.6 ± 0.2% and host cell DNA removal efficiency was ranging from 76.4% to 99.9% and strongly depended on pretreatment with deoxyribonuclease.
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Authors
Marko Banjac, Elisabeth Roethl, Franz Gelhart, Petra Kramberger, Barbara Lah Jarc, Marko Jarc, Aleš Štrancar, Thomas Muster, Matjaž Peterka,