| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10965193 | Vaccine | 2014 | 4 Pages |
Abstract
For this purpose, construction of recombinant baculoviruses was performed and viral stocks of BacRep, BacCap for serotypes 2, 5 and 6 were prepared in Sf9 cells. The recombinant baculovirus coding for the truncated capsid protein of HEV (BacITRHEVORF2) was also constructed, the virus titer was equal to 5.41Â ÃÂ 109Â PFU/mL, at the third passage. Transduction of HEK 293 EBNA cells with rAAV was carried out; the production of HEVORF2 was confirmed by Western blot. Optimization of rAAV production in Sf9 cells is currently ongoing.
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Authors
Khaled Trabelsi, Amine Kamen, Héla Kallel,