| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10967347 | Vaccine | 2012 | 8 Pages |
Abstract
⺠A simple strategy to formulate DC targeted PLGA nanoparticles (NPs) is reported. ⺠Ovalbumin-loaded biotinylated NPs were formulated using biotin-PEG-PLGA polymer. ⺠A recombinant bifunctional fusion protein (bfFp) is used as a DC targeting ligand. ⺠BfFp with OVA loaded NPs yields a versatile two-component antigen delivery system. ⺠DC targeted formulations along with αCD40 significantly enhance immune responses.
Keywords
APCPBS with Tween 20BsMAbBifunctional fusion proteinDEC-205HRPOCLRTMBPLGAOVAPBSTDLSscFvIMACMFI3,3′,5,5′-tetramethylbenzidinesingle chain antibodyAntigen presenting cellsOvalbuminVaccine deliverychicken ovalbuminEnzyme-linked immunosorbent assayELISARoom temperatureDendritic cellsmean fluorescence intensityPLGA nanoparticlesnanogramAntigenPSGHorseradish peroxidaseDynamic Light ScatteringPoly(d,l-lactic-co-glycolic acid)immobilized metal affinity chromatographyC-type lectin receptor
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Authors
Dharmendra Raghuwanshi, Vivek Mishra, Mavanur R. Suresh, Kamaljit Kaur,