| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10967950 | Vaccine | 2012 | 8 Pages |
Abstract
⺠The assessment of T cell responses is technically complex and usually performed on fresh PBMC. ⺠To identify simplified methods to assess pertussis specific T cell responses. ⺠High concordance in proliferation results was obtained when CFSE and blast read-outs were compared. ⺠Pertussis-specific assays performed on fresh PBMC were compared to frozen PBMC from the same donor. ⺠Results from fresh and frozen PBMC did not show significant difference. ⺠Adopting blast analysis and frozen PBMC, the possibility to test T cell responses is simplified.
Keywords
3H-Thymidine incorporationFBSPBMCFSCCPMSSCFHAPBSCFSEstandard error of the meancluster differentiationfoetal bovine serumCryopreservationperipheral blood mononuclear cellspertussis toxinPertussisProliferation indexcounts per minutePhosphate buffered salineSEMFrozenFilamentous hemagglutininT cell responsesforward scatterside scatter
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Authors
Maria Carollo, Raffaella Palazzo, Manuela Bianco, Kaatje Smits, Françoise Mascart, Clara Maria Ausiello,