Foot-and-mouth disease marker vaccine: Cattle protection with a partial VP1 G-H loop deleted virus antigen

► We have demonstrated that the VP1 G-H loop of FMDV is not necessary for protection in cattle. ► We have demonstrated that an FMD virus lacking the VP1 G-H loop can be used as an effective marker vaccine. ► We demonstrate that subclinical infection can be easily and rapidly diagnosed in cattle vaccinated with this negative marker vaccine using our associated diagnostic test.