Foot-and-mouth disease virus particles inactivated with binary ethylenimine are efficiently internalized into cultured cells

► Properties of chemically inactivated foot-and-mouth disease virus (FMDV) particles used for conventional vaccine production were characterized. ► Inactivated particles retained virion architecture and receptor binding properties. ► Inactivated particles were internalized into cells following an integrin dependent pathway. ► Inactivated particles were transported to early endosomes. ► Inactivated particles were transported to two different early endosome populations.