Article ID Journal Published Year Pages File Type
10969314 Vaccine 2011 11 Pages PDF
Abstract
The ultimate success of cancer vaccination is dependent upon the generation of tumor-specific CTLs. In this study, we designed and evaluated a novel fusion protein comprising a cell penetrating and immunostimulatory peptide corresponding to residues 32-51 of the Limulus polyphemus protein (LALF32-51) linked to human papillomavirus (HPV) 16 E7 antigen (LALF32-51-E7). We demonstrated that LALF32-51 penetrates the cell membrane and delivers E7 into cells. In a preclinical model of HPV16-induced cervical carcinoma we showed that vaccination with adjuvant-free LALF32-51-E7 fusion protein significantly improves the presentation of E7-derived peptides to T-cells in vitro and induces suppression of tumor growth.
Related Topics
Life Sciences Immunology and Microbiology Immunology
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