Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10969672 | Vaccine | 2010 | 11 Pages |
Abstract
Cell culture processes offer an attractive alternative to conventional chicken egg-based influenza vaccine production methods. However, most protocols still rely on the use of adherent cells, which makes process scale-up a challenging issue. In this study, it is demonstrated that the HEK-293 human cell line is able to efficiently replicate influenza virus. Production in serum-free suspension of HEK-293 cultures resulted in high titers of infectious influenza viruses for different subtypes and variants including A/H1, A/H3 and B strains. After virus adaptation and optimization of infection conditions, production in 3-L bioreactor resulted in titers of up to 109Â IVP/mL demonstrating the scale-up potential of the process.
Related Topics
Life Sciences
Immunology and Microbiology
Immunology
Authors
Audrey Le Ru, Danielle Jacob, Julia Transfiguracion, Sven Ansorge, Olivier Henry, Amine A. Kamen,