Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10970272 | Vaccine | 2009 | 11 Pages |
Abstract
The CAM-70 measles virus (MV) vaccine strain is currently used for vaccination against measles. We examined the fusion-inducing ability of the CAM-70 hemagglutinin (H) protein and found that it was impaired in both CD46- and signaling lymphocyte activation molecule (SLAM)-expressing cells. We also generated recombinant MVs possessing H genes derived from the CAM-70 strain. The CAM-70 H protein impaired viral growth in both CD46- and SLAM-expressing cells. In peripheral blood lymphocytes (PBL) and monocyte-derived dendritic cells (Mo-DC), the CAM-70 strain did not grow efficiently. Infection with recombinant MVs revealed that impaired growth of the CAM-70 strain was attributed to the H gene only partly in PBL and largely in Mo-DC. Thus, impaired fusion-inducing ability of the H protein may be one of the underlying molecular mechanisms resulting in the attenuation of the CAM-70 strain.
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Authors
Seiichi Kato, Shinji Ohgimoto, Luna Bhatta Sharma, Sekiko Kurazono, Minoru Ayata, Katsuhiro Komase, Makoto Takeda, Kaoru Takeuchi, Toshiaki Ihara, Hisashi Ogura,