Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10971445 | Developmental & Comparative Immunology | 2014 | 5 Pages |
Abstract
Mannose receptor C type 1 (MRC1) is a pattern-recognition receptor (PRR) which plays a significant role in immune responses. Much work on MRC1 has been done in mammals and birds while little in fish. In this study, we cloned and characterized MRC1 in grass carp (gcMR). The full-length gcMR contained 5291 bp encoding a putative protein of 1432 amino acids. The predicted amino acid sequences showed that gcMR contained a signal peptide, a cysteine-rich (CR) domain, a fibronectin type II (FN II) domain, eight C-type lectin-like domains (CTLDs), a transmembrane domain and a short cytoplasmic domain. gcMR were constitutively expressed in different organs with the higher expression in spleen and head kidney. During embryonic development, gcMR transcript levels were highest at cleavage stage. The up-regulation expression of gcMR, IL-1β and TNF-α in liver, spleen, head kidney and intestine after Aeromonas hydrophila infection indicating it involved in innate immune regulation during bacterial infections.
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Authors
Li Wang, Lichun Liu, Yang Zhou, Xiaoheng Zhao, Mingjun Xi, Shun Wei, Rui Fang, Wei Ji, Nan Chen, Zemao Gu, Xueqin Liu, Weimin Wang, Muhammad Asim, Xiaoling Liu, Li Lin,