Article ID Journal Published Year Pages File Type
10971642 Developmental & Comparative Immunology 2012 9 Pages PDF
Abstract
► Drosophila melanogaster three prophenoloxidases (PPOs) can be expressed in Escherichia coli with different enzyme activity. ► Protein expression conditions: at 16 °C and with Cu2+ added. ► Three rPPOs can be activated by serine protease. ► rPPO1 and rPPO3 have high Cu2+ association capability.
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Life Sciences Biochemistry, Genetics and Molecular Biology Developmental Biology
Authors
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