Large-scale RNAi screens add both clarity and complexity to Drosophila NF-κB signaling

►NF-κB signaling is an immune response mechanism remarkably conserved through phylogeny. ►The genetically tractable model animal Drosophila melanogaster has been an important model organism for studying NF-κB signaling. ►Fruit flies have two NF-κB signaling pathways: the Toll and the Imd pathway. ►Genome-wide RNAi -based screening has become a common method for identifying novel genes required for NF-κB signaling. ►These screens have helped us to understand better the immune response also in humans.