Channel catfish leukocyte immune-type receptor mediated inhibition of cellular cytotoxicity is facilitated by SHP-1-dependent and -independent mechanisms

Article ID	Journal	Published Year	Pages	File Type
10971725	Developmental & Comparative Immunology	2012	13 Pages	PDF

Abstract

âº IpLITRs encoding cytoplasmic ITIMs are potent inhibitors of lymphocyte cytotoxicity. âº IpLITR-mediated inhibition is mediated by a SHP-1-dependent and -independent mechanism. âº The SHP-1-independent inhibitory mechanism is also ITIM-independent and may involve binding of the C-terminal Src kinase. âº Teleosts contain an array of inhibitory signaling mediators and their intracellular targets similar to those found in mammals.

Keywords

Teleosts IL-2 PAB PI3-K SFK D-PBS PEI ITAM RVV MHC I FcRs SH2 IgSF Phosphatidylinositol 3-Kinases CSK Leukocyte immune-type receptors EAT-2 SIRP SH2D1A Signal-regulatory protein C-terminal Src kinase immune receptor tyrosine-based activation motif mAb HRP SHP kDa kiloDalton natural killer E:T KIRs MOI Monoclonal antibody Polyclonal antibody Human leukocyte antigen HLA interleukin 2 Syk Immunoprecipitation Tyrosine Immunoglobulin superfamily Extracellular domains lymphokine activated killer cells Natural killer cells Cytotoxicity cyt Cytoplasmic tail spleen tyrosine kinase dominant-negative phycoerythrin transmembrane Dulbecco’s phosphate buffered saline Phenylalanine LAK lymphokine activated killer hemagglutinin Src Homology 2 vaccinia virus recombinant vaccinia virus Horseradish peroxidase Polyethylenimine Chk multiplicity of infection Chromium SHIP Major Histocompatibility Class I Truncated Channel catfish Fc receptors killer cell immunoglobulin-like receptors Chimeric receptors