Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10971740 | Developmental & Comparative Immunology | 2007 | 11 Pages |
Abstract
Ovine MX1 (MX1) is expressed in the uterus during the estrous cycle and is strongly up-regulated during early pregnancy in the uterus and peripheral blood leukocytes. In this study we cloned the MX1 gene promoter/enhancer, and tested its response to interferon tau (IFN-tau). To address the role of IFN tau in regulating MX1 expression, serial deletion mutants were prepared along with a clone that contained a full-length promoter including the two proximal ISREs but lacking an intronic ISRE site. Promoter deletions showed the two proximal ISRE sites, but not the intronic ISRE site, were required for maximal response to IFN tau. Interestingly, MX1 promoter deletion mutants revealed the presence of distal positive (â920 to â715) and negative (â715 to â437) regulatory regions. Identifying positive and negative regulatory regions in MX1 promoter will help define the complex regulation of MX1 during early pregnancy in ruminants.
Keywords
ISGPGFNF-kappaBIRFGTPaseAP-1SRFOTRISRETSS5′RACEOGENF-κB5′ Untranslated region5′UTRMx1Sp1STATEndometriuminterferoninterferon stimulated response elementIFNinterferon stimulated genePregnancyOvinecorpus luteumtranscription start siteAntiviralIFN regulatory factorTranscription factoractivator protein-1PromoterJAKGasEstrogen receptoroxytocin receptorProgesterone receptor
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Authors
A.M. Assiri, T.L. Ott,