Tetraspanin-2 localisation in high pressure frozen and freeze-substituted Schistosoma mansoni adult males reveals its distribution in membranes of tegumentary vesicles

- Adult schistosomes are prepared for use with immuno-electron microscopy cryopreparation techniques.
- The method produces material of high structural integrity enabling molecular localisation.
- The method was used to define the subcellular location of SmTSP-2, an efficacious vaccine target.
- SmTSP-2 is commonly found in membranous vesicles of the tegument but not the apical membrane complex.