Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10972880 | International Journal for Parasitology | 2008 | 7 Pages |
Abstract
Gene expression in Trypanosomatids requires processing of polycistronic transcripts to generate monocistronic mRNAs by cleavage events that are coupled to the addition of a Spliced Leader sequence (SL) at the 5â²-end and a poly(A) tail at the 3â²-end of each mRNA. Here we investigate the sequence requirements involved in Trypanosoma cruzi mRNA processing by mapping all available expressed sequence tags and cDNAs containing poly(A) tail and/or SL to genomic intergenic regions. Amongst other parameters, we determined that the median lengths of 5â² untranslated region (UTR) and 3â²UTR sequences are 35 and 264 nucleotides, respectively; and that the median distance between SL addition sites and a polypyrimidine motif is 18 nucleotides, whereas the median distance between poly(A) addition sites and the closest polypyrimidine-rich sequence is 40 nucleotides.
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Authors
Priscila C. Campos, Daniella C. Bartholomeu, Wanderson D. DaRocha, Gustavo C. Cerqueira, Santuza M.R. Teixeira,