Article ID Journal Published Year Pages File Type
10972937 International Journal for Parasitology 2008 12 Pages PDF
Abstract
Theileria parva causes an acute lympho-proliferative disease in cattle, which can result in death of susceptible animals within 2-3 weeks of infection. Analyses of the cellular response in the lymph node draining the site of infection demonstrated an early T cell response, with the appearance of large numbers of uninfected lymphoblasts between 6 and 9 days p.i., coinciding with initial detection of parasitised cells. There was a marked increase in the representation of CD8+ T cells and the emergence of a sizable sub-population of CD2− CD8+ α/β T cells during this period. Analysis of T cell receptor β chain variable (TCR BV) gene expression did not reveal any evidence for the involvement of a superantigen in stimulating the response. Responding lymph node cells were found to produce increased quantities of IFNγ and IL-10, and both the CD2+ CD8+ and CD2− CD8+ populations expressed IFNγ transcripts. Purified CD2+ CD8+ cells proliferated when stimulated in vitro with autologous parasitised cells or non-specific mitogens, whereas CD2− CD8+ cells were refractory to these stimuli. In contrast to the parasite-specific cytotoxic activity associated with T cell responses in immune cattle, the responses to primary infection exhibited variable levels of non-specific cytotoxic activity. Stimulation of purified CD2+ CD8+ T cells in vitro with autologous parasitised cells also failed to reveal evidence of specific cytotoxic activity. These findings indicate that primary infection with T. parva induces an aberrant T cell response that lacks appropriate effector activity.
Related Topics
Life Sciences Immunology and Microbiology Parasitology
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