Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
11005641 | Food Chemistry | 2019 | 34 Pages |
Abstract
β-Agarase was immobilized by using tannic acid modified-Fe3O4 magnetic nanoparticles (TA-MNPs) as a support material. The MNPs were synthesized by improved chemical coprecipitation method and modified with TA for agarase immobilization. TA-MNPs and immobilized β-agarase were characterized by transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), and thermal gravimetric analysis (TGA), all of which indicated the successful surface modification of MNPs with TA and the immobilization of β-agarase. The optimal immobilization conditions for 25â¯mg TA-MNPs included 100â¯r/min oscillation speed, immobilization time of 2â¯h, immobilization temperature of 15â¯Â°C, and initial β-agarase concentration of 3â¯mL (480â¯U). Immobilized β-agarase showed better pH and thermal stability and excellent reusability than the free enzyme. Results revealed the promising application of β-agarase-TA-MNPs for the preparation of neoagaro-oligosaccharides with different average polymerization degrees and varying activities in the antioxidant.
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Authors
Qiong Xiao, Chunli Liu, Hui Ni, Yanbing Zhu, Zedong Jiang, Anfeng Xiao,