Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
11005727 | Journal of Trace Elements in Medicine and Biology | 2018 | 9 Pages |
Abstract
The study was conducted to investigate the effects of different circadian calcium feeding regimes on parameters of Zn status and gene expression of circadian clock, calcium transporters and oxidative status in laying hens. In total, 180 of 41-weeks Brown Hy-line laying hens were assigned randomly into three groups, 1-CON group (Control Ca, diets contained 3.4% Ca at both 0730 and 1530â¯h), 2-HL group (High-low Ca, diets contained 3.6%-3.2% Ca respectively) and 3-LH group (Low-high Ca, diets contained 3.2%-3.6% Ca respectively), which were fed a certain amount of control diet at 0730â¯h and 1530â¯h. Blood, tibia, jejunum and kidney samples were collected at 4â¯h intervals with initial starting at 0800â¯h after 10 weeks of experiment. Compared with the CON group: 1) the serum zinc in HL group increased at 2000â¯h, but lower at 1600â¯h in LH group (Pâ¯<â¯0.05). 2) in jejunum, circadian clock genes including CLOCK and BMAL1 expression of HL group were down-regulated at 0000â¯h and 1600â¯h, as well as CLOCK, BMAL1, Cry2, Per3 and calcium transporter gene NCX1 in LH group at 2000â¯h (Pâ¯<â¯0.05). 3) in kidney, CLOCK, Cry1, Cry2 and Per3 of LH group were up-regulated at 0400â¯h, CLOCK at 0000â¯h as well, while CLOCK at 2000â¯h were down-regulated (Pâ¯<â¯0.05). 4) in kidney, the calcium transporters including PMCA, CaBP and CA of LH group were up-regulated at 0000â¯h, but PMCA and CaBP of LH group were down-regulated at 0800â¯h, 1200â¯h and 1600â¯h, as well as CA at 1600â¯h and PMCA at 2000â¯h of LH group (Pâ¯<â¯0.05), and the oxidative gene SOD of the LH group was up-regulated at 0400â¯h, as well as CAT at 0400â¯h, SOD and GPX1 at 1200â¯h in HL group, but SOD at 1600â¯h and 2000â¯h, and GPX1 at 1600â¯h were down-regulated in LH group (Pâ¯<â¯0.05). These results demonstrated that the dynamic circadian calcium feeding regime affected circadian rhythms of serum zinc concentration as well as the expression of certain genes related to the circadian clock, calcium transport and antioxidative capacity, and circadian calcium feeding regimes may therefore be considered with regard to improving the calcium usability.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Xue Lin, Yilin Liu, Tiantian Meng, Chunyan Xie, Xin Wu, Yulong Yin,