Polyglycerol sebacate/chitosan/gelatin nano-composite scaffolds for engineering neural construct

The aim of this study was designing and fabricating nano-composite scaffolds based on polyglycerol sebacate in order to use in nerve tissue engineering. Semi-crystalline polyglycerol sebacate polymer was synthesized and evaluated by X-ray diffraction (XRD) and Fourier-transform infrared spectroscopy (FTIR) methods. The synthesized polyglycerol sebacate was electrospun with chitosan and gelatin at different concentrations under various conditions. The obtained samples were examined using Scanning electron microscopy (SEM) observations; the diameters of fibers were measured via image processing method, and the percentage of scaffolds porosity were obtained as well. Results demonstrated that semi-crystalline polyglycerol sebacate/chitosan/gelatin could be electrospun to produce fibers with the diameter of about 80 nm in average. MTT assay were performed using PC12 cell line; after 3 days of cell culture, it seems that polyglycerol sebacate/Chitosan/Gelatin nano-composite has potentiality for nerve tissue engineering applications.