Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
11016073 | Journal of Chromatography A | 2018 | 11 Pages |
Abstract
A sensitive, simple and rapid method for the simultaneous determination of eleven arsenic species has been developed. A high performance liquid chromatography-inductively coupled plasma mass spectrometry (HPLC-ICP-MS) technique was used for the analysis of eleven arsenic species in less than 17âmin. Different extraction solutions were explored and the recovery results using water and aqueous acidic solvents, aqueous organic solvents and enzymes showed that 40âmg protease with 0.75âmL 0.5% hydrochloric acid (v/v) as the extraction agent gave the best experimental results. Species separation with ammonium carbonate solution as the mobile phase was conducted on an anion-exchange chromatographic column using gradient elution. The column temperature was 20â°C and kinetic energy discrimination (KED) was employed to eliminate spectral interference. The use of KED mode effectively removed interference from 75ArCl. The detection limit (LD) of the method was in the range of 0.11-0.59âμg kgâ1. Repeatability values obtained for spiked real fish samples were in the range of 1.1%-7.6%. Accuracy was calculated based on the analysis of spiked real fish samples at five concentration levels. Obtained recoveries were 91%-106%. The validated method was used in a pilot study to analyze real samples of fish, the organic arsenic especially AsB was the major arsenic specie present in the analyzed samples, only trace amount of inorganic arsenic were detected. The analytical method should improve the assessment of human exposure associated with arsenic intake from fish.
Keywords
MMAIARCArsenocholineKEDArsenobetaineROXASCHPLC-ICP-MSDMAASAInternational Agency for Research on CancerEuropean Uniondimethylarsinic acidmonomethylarsonic acidKinetic energy discriminationDetection limitNITfood and agricultural organizationFAOASBFishQuantification limitNAPPHeliumProteaseAmmonium carbonateArsenic species
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Fa Zhao, Yanming Liu, Xiqi Zhang, Rui Dong, Wenjiang Yu, Yanfang Liu, Zhimou Guo, Xinmiao Liang, Jianhua Zhu,