| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 11017300 | Talanta | 2019 | 24 Pages |
Abstract
We developed a novel method for efficiently screening aptamers targeted lactoferrin from a complex ssDNA library based on silver decahedral nanoparticles (AgNP) and fluorescence activated cell sorting (FACS). Six aptamers (Ylac1, Ylac4, Ylac5, Ylac6, Ylac8 and Ylac9) were obtained after five-round of selection, which showed good specificity towards lactoferrin. And the equilibrium dissociation constants (Kd) of six aptamers were calculated and all were in the nanomolar range.167
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Fang Yu, Hui Li, Wei Sun, Yaju Zhao, Danke Xu, Fuchu He,