Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
11023205 | Analytical Biochemistry | 2018 | 6 Pages |
Abstract
Excess urinary orotic acid excretion occurs in patients with some inborn errors of metabolic pathways such as pyrimidine synthesis and urea cycle. Thus, rapid diagnosis of orotic aciduria has a vital importance for patients. In this paper, a novel method for determination of orotic acid in urine samples by ion chromatography with suppressed conductivity detection was investigated. The separation of orotic acid from urine matrix was accomplished by using an anion exchange column with optimized isocratic eluent program which utilized 50â¯mM NaOH. The other chromatographic conditions were as follows: the suppressor current was 31â¯mA; the flow rate of mobile phase was 0.25â¯mLâ¯minâ1; the column temperature was 30â¯Â°C; sample loop volume was 10â¯Î¼L. Under optimized conditions, the limit of detection was 0.2â¯Î¼molâ¯Lâ¾1. Dramatically elevated orotic acid concentration was observed on pathological urine samples comparing to healthy urines, as expected. There are a good many advantages of the proposed method, but using an environmentally friendly reagent free system, no organic solvent employment and its quick nature, and being a sensitive and reliable method are the most obvious ones. The proposed method, therefore, may be utilized as an alternative technique for clinical laboratories.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Orhan DestanoÄlu, Damla Zeydanlı, Mehmet Åerif Cansever, Gülçin GümüÅ Yılmaz,