Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
11025647 | Toxicology in Vitro | 2019 | 30 Pages |
Abstract
Prostate cancer is the most common malignancy in men. Phthalate esters are a class of environmental endocrine disruptors and were reported to be cancer promoting agents, however the potential role of phthalate esters in prostate cancer has been rarely reported. Mounting evidence has shown that miR-34a is a master tumor suppressor miRNA in cancer. The aim of this study was to investigate the role of butyl benzyl phthalate (BBP), one of the typical phthalate esters, in cell proliferation of prostate cancer cells. Human prostate cancer LNCaP and PC-3 cells were exposed to low dose of BBP for 6â¯days. The results showed that 10â6 and 10â7â¯mol/L BBP increased the expression of cyclinD1 and PCNA, decreased p21 expression, and induced cell growth in both LNCaP and PC-3 cells. Furthermore, we found that BBP significantly downregulated the expression of miR-34a, along with upregulation of miR-34a target gene c-myc. Using cell tranfection of miR-34a mimic and inhibitor, we demonstrated that BBP promoted cell proliferation through miR-34a/c-myc axis in prostate cancer cells. Findings from this study could provide new insight into the involvement and the molecular mechanism of phthalate esters on prostate cancer.
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Authors
Mingming Zhu, Jieshu Wu, Xiao Ma, Cong Huang, Rui Wu, Weiwei Zhu, Xiaoting Li, Zhaofeng Liang, Feifei Deng, Jianyun Zhu, Wei Xie, Xue Yang, Ye Jiang, Shijia Wang, Shanshan Geng, Chunfeng Xie, Caiyun Zhong,