Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
11025681 | Chemico-Biological Interactions | 2018 | 46 Pages |
Abstract
Fasiglifam (TAK-875), a G protein-coupled receptor 40 (GPR40) agonist, was a drug candidate for type 2 diabetes. However, its development was terminated in phase 3 trials due to liver safety concerns. Although TAK-875 was reported to inhibit hepatobiliary transporters and disturb bile acid disposition, pathogenic mechanisms of TAK-875-induced liver injury are not fully understood. In this study, we sought to identify the mechanisms with a hepatic genome-wide transcriptomic analysis in a murine model. We demonstrated that, among the three GPR40 agonists, TAK-875, AMG-837, and TUG-770, only TAK-875 induced acute liver injury in mice. Transcriptome profiles of TAK-875-exposed liver was compared with those of non-hepatotoxic analogues AMG-837 and TUG-770 as negative controls and those of classical hepatotoxicants concanavalin A and carbon tetrachloride as positive controls. The comparative hepatic transcriptome analyses revealed the enrichment of genes involved in inflammation, endoplasmic reticulum (ER) stress, apoptosis, and hepatic lipid accumulation, suggesting that these events play pathophysiologic roles in the development of TAK-875-induced liver injury. These results were validated by quantitative PCR with significant changes in chemokines, danger signals, ER stress mediators, proapoptotic factors, and hepatic steatosis markers only in TAK-875-exposed liver. Pretreatment of TAK-875-administered mice with an ER stress inhibitor 4-phenylbutyric acid (4-PBA) alleviated the liver injury. Consistent with the in vivo study, pretreatment of HepG2 cells with 4-PBA significantly improved the decrease of cell viability induced by TAK-875. In conclusion, by a comprehensive transcriptomic analysis, we found multiple possible processes that contribute to TAK-875-induced acute liver injury in mice.
Keywords
nESOATPsFFAR4-PBAConANtcpNa+-taurocholate co-transporting polypeptideDrug-induced liver injury (DILI)BSEPMrp2TLRCCl4ALTGPRConcanavalin ADAMPsFDRDEXG-protein coupled receptorDrug-induced liver injuryAlanine aminotransferase4-phenylbutyric aciddamage-associated molecular patternsinterleukinGene Set Enrichment AnalysisToll-like receptorDexamethasoneDILIMicroarrayendoplasmic reticulumDEGfalse discovery ratenormalized enrichment scoreGene ontologymultidrug resistance-associated protein 2Organic anion transporting polypeptidesBile salt export pumpDifferentially expressed geneCarbon tetrachlorideHPLChigh-performance liquid chromatographyfree fatty acid receptor
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Authors
Yuya Urano, Shingo Oda, Koichi Tsuneyama, Tsuyoshi Yokoi,