Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
11025686 | Chemico-Biological Interactions | 2018 | 7 Pages |
Abstract
Phloretin (Phl) is a dihydrochalcone flavonoid with significant cytoprotective properties; e.g., free radical trapping, electrophile scavenging. Based on this, it has been suggested that Phl might be useful in the treatment of pathogenic processes and prevention of drug toxicities. Therefore, we determined the ability of Phl to provide route- and dose-dependent hepatoprotection in a mouse model of acetaminophen (APAP) overdose. Intraperitoneal (i.p.) administration of Phl produced a bimodal effect; i.e., the highest dose (2.40â¯mmol/kg) did not prevent APAP-induced lethality, whereas lower doses (0.2-0.4â¯mmol/kg) afforded modest hepatoprotection. When given alone, the highest i.p. Phl dose was lethal within 24â¯h, whereas the lower doses were not toxic. Oral Phl (0.40-2.40â¯mmol/kg) did not prevent APAP-induced hepatotoxicity. The highest oral dose given alone (2.4â¯mmol/kg) produced 64% lethality, whereas lower doses were not lethal. This toxicity profile was reflected in a study using APAP-exposed isolated mouse hepatocytes, which showed that the Phl pharmacophores, 1,3,5-trihydroxyacetophenone (PG) and 2â²,4â²,6â²-trihydroxyacetophenone (THA) where protective. Corroborative cell free studies showed that polyphenol protectants prevented glutathione loss mediated by the APAP metabolite, N-acetyl-p-benzoquinone imine (NAPQI). Thus, in spite of possessing cytoprotective attributes, Phl was generally toxic in our APAP models. These and earlier findings suggest that Phl is not a candidate for drug design. In contrast, we have found that the enol-forming pharmacophores, THA and PG, are potential platforms for pharmacotherapeutic development.
Keywords
Related Topics
Life Sciences
Environmental Science
Health, Toxicology and Mutagenesis
Authors
Brian C. Geohagen, Boris Korsharskyy, Amaresh Vydyanatha, Lars Nordstroem, Richard M. LoPachin,