Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
11026862 | Materials Science and Engineering: C | 2019 | 39 Pages |
Abstract
In the present study we report about an in vitro model of adipose-derived stem cells (ADSCs) loaded with palmitate-coated MNPs (MNPsPA) as antitumor drug carriers targeting a 3D tissue-like osteosarcoma cells. Cell viability, MNPsPA-drug loading capacity, cell speed, drug release rate, magnetization and zeta potential were determined and analysed. The results revealed that ADSCs loaded with MNPsPA-drug complexes retained their viability at relatively high drug concentrations (up to 1.22â¯pg antitumor drug/cell for 100% cell viability) and displayed higher speed compared to the targeted tumor cells in vitro. The magnetization of the sterilized MNPsPA complexes was 67â¯emu/g within a magnetic field corresponding to induction values of clinical MRI devices. ADSCs payload was around 9â¯pg magnetic material/cell, with an uptake rate of 6.25â¯fg magnetic material/min/cell. The presented model is a proof-of-concept platform for stem cells-mediated MNPs-drug delivery to solid tumors that could be further correlated with MRI tracking and magnetic hyperthermia for theranostic applications.
Related Topics
Physical Sciences and Engineering
Materials Science
Biomaterials
Authors
Dumitru-Daniel Herea, Luminita Labusca, Ecaterina Radu, Horia Chiriac, Marian Grigoras, Oana Dragos Panzaru, Nicoleta Lupu,