Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
11027673 | Biosensors and Bioelectronics | 2018 | 25 Pages |
Abstract
An improved amperometric sarcosine biosensor was constructed based on covalent immobilization of sarcosine oxidase nanoparticles (SOxNPs) onto gold electrode (AuE). The SOxNPs/AuE was characterized by scanning electron microscopy (SEM), fourier transform infrared (FTIR) spectroscopy and electrochemical impedance spectroscopy (EIS) at different stages of its construction. The biosensor worked optimally within 2â¯s at a potential of 1.0â¯V, against Ag/AgCl, pH 6.5 and 35â¯Â°C. A linear relationship was observed between sarcosine concentration range, 0.1-100â¯Î¼M and the biosensor response i.e. current in mA under optimum conditions. The biosensor offered a low detection limit of 0.01â¯Î¼M and gratifying storage stability. The SOxNPs/AuE was unaffected by a number of serum substances at their physiological concentrations. The biosensor measured sarcosine level in sera collected from persons suffering from prostate cancer (mean13.5â¯Î¼M, nâ¯=â¯8), which was significantly higher (pâ¯<â¯0.01) than those in apparently healthy persons (mean 2.2â¯Î¼M, nâ¯=â¯8). The SOxNPs/Au electrode was reused 300- times during the span of 180 days, with only 10% loss in its initial activity while being stored dry at 4â¯Â°C.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Parveen Kumar, Vinay Narwal, Ranjana Jaiwal, C.S. Pundir,