Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
11029058 | Virology | 2018 | 10 Pages |
Abstract
Genus Gammapapillomavirus (Gamma-PV) is the most diverse and largest clade within the Papillomaviridae family. A novel set of degenerate primers targeting the E1 gene was designed and further used in combination with the well-known CUT PCR assay to assess HPV prevalence and genus distribution in a variety of cutaneous samples from 448 immunocompetent individuals. General HPV, Gamma-PV and mixed infections prevalence were significantly higher in actinic keratosis with respect to benign and malignant neoplasms, respectively (pâ¯=â¯0.0047, pâ¯=â¯0.0172, pâ¯=â¯0.00001). Gamma-PVs were significantly more common in actinic keratosis biopsies than Beta- and Alpha-PVs (pâ¯=â¯0.002). The full-length genome sequence of a novel putative Gamma-PV type was amplified by 'hanging droplet' long-range PCR and cloned. The novel virus, designated HPV210, clustered within species Gamma-12. This study provides an additional tool enabling detection of HPV infections in skin and adds new insights about possible early roles of Gamma-PVs in the development of cutaneous malignant lesions.
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Authors
Elisa M. Bolatti, Lea HoÅ¡njak, Diego Chouhy, Maria F. Re-Louhau, Pablo E. Casal, Hebe Bottai, BoÅ¡tjan J. Kocjan, Emma J. Stella, Mario D. Gorosito, Adriana Sanchez, Ramón Fernandez Bussy, Mario Poljak, Adriana A. Giri,