Overexpression of Sirt1 in mesenchymal stem cells protects against bone loss in mice by FOXO3a deacetylation and oxidative stress inhibition

Article ID	Journal	Published Year	Pages	File Type
11031836	Metabolism	2018	34 Pages	PDF

Abstract

Taken together, these results demonstrate that Sirt1 overexpression in MSCs increased the osteoblastic bone formation and partially restores the defects in skeletal growth and osteogenesis in Bmi-1â/â mice by FOXO3a deacetylation and oxidative stress inhibition. Our data support the proposal that Sirt1 is a target for promoting bone formation as an anabolic approach for the treatment of osteoporosis.

Keywords

RUNX2 FOXO3a GSR TRAP GPx1 SOD1 MSC Bmi-1 Sirt1 RANKL SOD2 SA-β-gal sirtuin 1 TXN LPR OPG PRX1 OCN H&E ROS senescence associated β-galactosidase ALP Alkaline phosphatase Osteoprotegerin Osteocalcin tartrate-resistant acid phosphatase Immunoprecipitation Oxidative stress Micro-computed tomography thioredoxin forkhead Box O Deacetylation Mesenchymal stem cell superoxide dismutase 1 Superoxide dismutase 2 Runt-related transcription factor 2 FoxO Micro-CT NAM wild-type Nicotinamide Hematoxylin and Eosin Low-density lipoprotein receptor-related protein Col-I Type I collagen Type II collagen glutathione reductase glutathione peroxidase 1 Reactive oxygen species